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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Peripheral myelin protein-22 (PMP22) modulates alpha 6 integrin expression in the human endometrium

Figure 4

PMP22 and α6 integrin associate with each other. HEC-1A/Vector or HEC-1A/PMP22 cells were lysed in 1% NP-40 and incubated with PMP22 or α6 integrin antisera. Immunoprecipations were assessed by SDS-PAGE analysis. (A) α6 integrin and PMP22 co-immunoprecipitate in HEC-1A/Vector and HEC-1A/PMP22 cells when using either PMP22 or the α6 integrin antibody. Normal rabbit sera were used to assess nonspecific pull-down of proteins. (B) Confocal images of HEC-1A/PMP22 cells after co-immunolabeleing with anti-PMP22 (Rhodamine) and anti-α6 integrin (clone G0H3; FITC) antibodies. Co-localization of PMP22 protein (Rhodamine) with α6 integrin (FITC) was observed in both intracellular and plasma membrane compartments. Scale bar, 10 μM. Insets, 50 μM.

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