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Table 1 List of primers used for cloning and quantitative RT-PCR.

From: The spatiotemporal expression of multiple coho salmon ovarian connexin genes and their hormonal regulation in vitro during oogenesis

Step Gene name Strand Sequence Product size (bp)
cloning cx30.9 Sense 5'-GAAATTCCGAGGAGGTGGTC-3' 1,088
   Antisense 5'-GCATGTTTGTCCAGGACTAC-3'  
cloning cx34.3 Sense 5'-CTGAGAGACTGAGAGCTCCAAAC-3' 1,038
   Antisense 5'-AGACTGGCATGCGAATCATTG-3'  
cloning cx43.2 Sense 5'-TCAAGAAGTGACGGAGAAAG-3' 1,278
   Antisense 5'-GTACTCTCTGTTCTCTGGCA-3'  
cloning cx44.9 Sense 5'-AGCCTTAAAGAGCTGAGAGG-3' 1,273
   Antisense 5'-CGAGCAGGCATTAAGCAGGA-3'  
qPCR* cx30.9 Antisense 5'-GGCTGGTGGAGTGTTTGTTC-3' 99
qPCR cx34.3 Sense 5'-ACTACCTGTATGGCTTCACCCT-3' 284
   Antisense 5'-CTGGATCATCTGGTCTTTGTTC-3'  
qPCR cx43.2 Sense 5'-ATGGCTGTTCCTCTCCCACT-3' 121
   Antisense 5'-CAGTTTTGCTCGTTGGCTTG-3'  
qPCR cx44.9 Sense 5'-GTGACAGAAGGCTCTGTGAAG-3' 305
   Antisense 5'-ACACCGAGATGGAGAATCTC-3'  
  1. *qPCR for cx30.9 was performed using cx30.9 cloning primer with the antisense qPCR primer for cx30.9.