Figure 5

Hormonal regulation of ovarian cx genes in vitro : culture experiment 2. Effects of varying LH (10, 50, 100, or 500 ng/ml) and IGF1 (1, 10, or 100 nM) concentrations on transcripts for cx34.3 (A, C) and cx43.2 (B, D) at the late vitellogenic (VIT)-stage. Data are expressed as the mean ± SEM (n = 6) and presented relative to the expression of controls (cont; Time 36 h, no hormones, set to 1). Bars not sharing the same letter are significantly different (P < 0.05). Asterisks denote values that significantly differ between initial (Time 0, no hormones) and control samples, **P < 0.01. ns, no significant difference.