Depletion of tNASP from HeLa and PC-3 cells decreases cell proliferation. Upper panel "tNASP content" (A, E, I) shows the distribution of tNASP depleted and tNASP containing cells after the tNASP shRNA treatment as determined by FASC analysis on day 6. Panel "Parental population" (B, F, J) shows the original position of labeled, non-dividing cells on day 3. Panels "Scrambled shRNA" (C, G, K) and "tNASP shRNA" (D, H, L) show the proliferation of cells after they were treated by tNASP shRNA or scrambled shRNA. Each peak shows the population of cells with diminished content of CFSE due to cell divisions. Data were collected on day 6. Only tNASP depleted cells were analyzed for proliferation, tNASP containing cells were gated out. Figure 5 is a representative result from a total of 3 experiments.