Northern blot analysis of total RNA from HeLa cells. Line 1: HeLa cells treated with tNASP shRNA; line 2: HeLa cells treated with scrambled shRNA; line 3: untreated HeLa cells. Blot was hybridized with the 750 bp sequence common to both tNASP and sNASP. Lower panel shows GAPDH loading control. Figure 1 is a representative result from a total of 3 experiments.