In-vitro effects of the antimicrobial peptide Ala8,13,18-magainin II amide on isolated human first trimester villous trophoblast cells

Table 2 Effect of AMA on time course characteristics of cellular behavior of placental villous CTB in vitro

Test parameter	Median value (ranges)
	Time
Treatment	24 h	48 h	96 h
Specific stain positivity in ICC (n = 6/each treatment sub-group)
β hCG ^a
Control	13.0 (8-18)	17.5* (11-27)	23.5^(*)(18-32)
AMA	11.0 (9-20)	13.0 (9-22)	17.0 (11-24)
hPL ^a
Control¹	9.5 (7-12)	5.5* (9-20)	21.0^(*)(14-30)
AMA	8.0 (6-13)	11.0 (8-18)	14.5 (10-21)
Cyto18 neo-epitope ^a
Control	2.5 (1-4)	2.5 (1-5)	4.5^[*](3-8)
AMA	3.5 (2-6)	3.5 (2-7)	8.5^(*)(5-11)
TUNEL ^b
Control	0 (0-3)	0 (0-2)	6^[*](3-9)
AMA	3 (0-5)	3 (1-7)	11^(*)(6-17)
Optical densityc in WB analysis (n = 5/each)
β hCG
Control	20.5 (11-27)	22.0 (12-28)	30.5^(*)(19-36)
AMA	18.0 (9-28)	21.5 (9-24)	22.5 (11-27)
hPL
Control	9.5 (6-12)	10.0 (7-18)	16.5^(*)
AMA	9.5 (7-13)	9.5 (6-18)	11.5 (9-21)
Cyto18 neo-epitope
Control	4.0 (2-5)	4.0 (2-6)	6.5^[*](3-11)
AMA	5.5 (2-7)	5.5 (2-8)	9.5^(*)(5-16)

^apositive cellular area in per cent. ^bnumber of positive cells in per cent. ^cintegrated optical density normalized by that of β-actin per 25 μg of protein.
^(*)P < 0.05 compared with any other group; ^[*]P < 0.05 compared with 24 h and 48 h groups in control treatment; *P < 0.05 compared with control treatment at 96 h.

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