Skip to main content

Table 2 Effect of AMA on time course characteristics of cellular behavior of placental villous CTB in vitro

From: In-vitro effects of the antimicrobial peptide Ala8,13,18-magainin II amide on isolated human first trimester villous trophoblast cells

Test parameter Median value (ranges)
  Time
Treatment 24 h 48 h 96 h
Specific stain positivity in ICC (n = 6/each treatment sub-group)
β hCG a
Control 13.0 (8-18) 17.5* (11-27) 23.5(*)(18-32)
AMA 11.0 (9-20) 13.0 (9-22) 17.0 (11-24)
hPL a
Control1 9.5 (7-12) 5.5* (9-20) 21.0(*)(14-30)
AMA 8.0 (6-13) 11.0 (8-18) 14.5 (10-21)
Cyto18 neo-epitope a
Control 2.5 (1-4) 2.5 (1-5) 4.5[*](3-8)
AMA 3.5 (2-6) 3.5 (2-7) 8.5(*)(5-11)
TUNEL b
Control 0 (0-3) 0 (0-2) 6[*](3-9)
AMA 3 (0-5) 3 (1-7) 11(*)(6-17)
Optical densityc in WB analysis (n = 5/each)
β hCG
Control 20.5 (11-27) 22.0 (12-28) 30.5(*)(19-36)
AMA 18.0 (9-28) 21.5 (9-24) 22.5 (11-27)
hPL
Control 9.5 (6-12) 10.0 (7-18) 16.5(*)
AMA 9.5 (7-13) 9.5 (6-18) 11.5 (9-21)
Cyto18 neo-epitope
Control 4.0 (2-5) 4.0 (2-6) 6.5[*](3-11)
AMA 5.5 (2-7) 5.5 (2-8) 9.5(*)(5-16)
  1. apositive cellular area in per cent. bnumber of positive cells in per cent. cintegrated optical density normalized by that of β-actin per 25 μg of protein.
  2. (*)P < 0.05 compared with any other group; [*]P < 0.05 compared with 24 h and 48 h groups in control treatment; *P < 0.05 compared with control treatment at 96 h.