Table 1 Primer and probe sequences used in qRT-PCR for PAPPA2, PAPPA, ADAM12, E-cadherin, β-actin and GAPDH

PAPPA2

ACTCACCCAAGAGGGCATACATGA

GCACTGAGCTGGCAAAGTAGATGT

50°C

This paper

PAPPA

GTCATCTTTGCCTGGAAGGGAGAA

AGGGCTGTTCAACATCAGGATGAC

56°C

This paper

ADAM12

CTGGGCCACAATTTCGGGATGAAT

ACTGCTGAACACCATGGGAAATGG

50°C

This paper

E-cadherin

AGCCTCTGGATAGAGAACGCATTG

GGGTGAATTCGGGCTTGTTGTCAT

50°C

This paper

β-actin

GCGAGAAGATGACCCAGGATC

CCAGTGGTACGGCCAGAGG

60°C

Knerr et al. (2005) [31]

GAPDH

CGGGAAGCTTGTGATCAATGG

GGCAGTGATGGCATGGACTG

56°C

Kudo et al. (2003) [30]