Western blot analysis of NPM2 protein expression in Hela cells transfected with NPM2 and miR-181a expression plasmids. A. A representative Western blot showing suppression of NPM2 protein in cells expressing miR-181a. B. Densitometric analysis of protein bands on the Western blots. Data were normalized to GAPDH and expressed as relative pixel density (mean ± SEM, n = 5). Different letters indicates statistical significance (P < 0.05).