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Table 2 Ultrastructural changes in IVG mouse ovarian follicles cultured with or without FSH (100 mIU/ml) for 6 days

From: Ultrastructure of isolated mouse ovarian follicles cultured in vitro

Group

N°

Shape

       
   

Oocyte

Granulosa

Theca

   

Organelle

distribution

Oolemma

Zona

Pellucida

N° and

arrangement of

layers

Transzonal

processes*

Signs of

degeneration

N° of

layers

FCS

20

Rounded

Juxta-nuclear

Microvilli +/-

Intact

4-5

Compact

++

Present in

40% of

samples

1-2

FSH

ANTRAL

20

Rounded

Scattered

Microvilli +

Intact

6-8

Cell loosening,

with fluid-filled

intercellular spaces

Innermost layer

discontinuous

+/-

Present in

10% of

samples

1-2

FSH

NON-

ANTRAL

20

Rounded

Scattered

Microvilli +/-

Intact

6-8

Compact

+

Present in

40% of

samples

1-2

  1. *See Table 1 for details