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Table 2 Ultrastructural changes in IVG mouse ovarian follicles cultured with or without FSH (100 mIU/ml) for 6 days

From: Ultrastructure of isolated mouse ovarian follicles cultured in vitro

Group Shape        
    Oocyte Granulosa Theca
    Organelle distribution Oolemma Zona Pellucida N° and arrangement of layers Transzonal processes* Signs of degeneration N° of layers
FCS 20 Rounded Juxta-nuclear Microvilli +/- Intact 4-5 Compact ++ Present in 40% of samples 1-2
FSH ANTRAL 20 Rounded Scattered Microvilli + Intact 6-8 Cell loosening, with fluid-filled intercellular spaces Innermost layer discontinuous +/- Present in 10% of samples 1-2
FSH NON- ANTRAL 20 Rounded Scattered Microvilli +/- Intact 6-8 Compact + Present in 40% of samples 1-2
  1. *See Table 1 for details