Table 1 Oligonucleotide sequences used for RT-PCR
Gene | Oligonucleotide sequences | Product size (bp) | GenBank/REFERENCES |
|---|---|---|---|
GAPDH | F 5'-CACCCTCAAGATTGTCAGCA-3' R 5'-GGTCATAAGTCCCTCCACGA-3' | 103 | BC102589 |
LTA4H | F 5'-CCCTAAAGAACTGGTGGCACT-3' R 5'-GACTTTTCCACCTGCTCTTTC-3' | 240 | NM00103428 [46] |
LTC4S | F 5'-CCTGCTGCAAGCCTACTTCT-3' R 5'-GTTCACTTGGGCTCGGTAGA-3' | 137 | NM001046098 |
PGES | F 5'-ATCGTGACGGTCCGTCTCTAA-3' R 5'-GCCCTTTGAGATTGTGACAGG-3' | 158 | NM174443 [47] |
PGFS | F 5'-TGTGGTGCACGTATCACGACA-3' R 5'-AATCACGTTGCCGTCCTCATC-3' | 169 | S54973 [47] |
EDN-1 | F 5'-CAAATGCATCCTGCCTGGTC-3' R 5'-ATTGCCACCCCCATAGAGGA-3' | 178 | X52740 [25] |
SV40 T-ag | F 5'-GCAATCGAAGCAGTAGCAATC-3' R 5'-CAGCTAATGGACCTTCTAGG-3' | 385 | SV40gp6 [48] |