Separation of protein Fraction C of llama seminal plasma. Separation was done using sephacryl gel filtration fast protein liquid chromatography (FPLC) and isocratic elution with phosphate buffered saline (left). Fraction C was isolated previously by hydroxylapatite gravity column chromatography. Vertical lines along the x-axis represent fractions collected and examined. The protein band at about 14 kDa on denaturing 12% SDS-PAGE (right) was the major constituent of Fraction C2 (right).