Figure 3

Immunoperoxidase for biglycan. (A) Ovx group: the immunoreaction is absent in all uterine tissues, being detected only in the cytoplasm of some leukocytes (arrowhead); (B) E2-group: the reaction is observed in the region of basement membrane of the luminal and glandular epithelia (arrows) and in the myometrium; (C) MPA-group: strong diffuse reaction in the whole endometrial stroma. Moderate immunoreaction is present in both layers of the myometrium and the connective tissue between them; (D) E2+MPA-group: the immunoreaction is distributed as a network of thin filaments in the whole stroma. In the myometrium, the deposition of biglycan is observed in both layers and connective tissue, being weak in the EML. L = uterine lumen; G = endometrial gland; SS = superficial stroma; DS = deep stroma; V = blood vessel; IML = internal muscle layer; EML = external muscle layer. Scale bar: 50 μm.