The effect of progesterone replacement on gene expression in the corpus luteum during induced regression and late luteal phase in the bonnet monkey (Macaca radiata)

Table 2 List of Primers used for real time RT-PCR analysis

S. No.	Gene name	Primer sequence (5'to 3')	Annealing temp (OC)	Product size (bp)
1	PR F	GCCACATTCAACACCCACTT	57.4	134
	PR R	CCTTCAGCTCAGTCATGACG
2	NCOA1 F	CTGCACGTGGGGGATCAT	60.4	146
	NCOA1 R	CCTGGCTCATCTGGAGGGT
3	NCOA2 F	CATGACCTCAGTGACCTCCGT	60.4	116
	NCOA2 R	CCATTCCAGGCAGCTGGTTT
4	NCOA3 F	CACCACAGGGCAGATGAGTG	60.4	135
	NCOA3 R	TGTGGGGGGCTACTCATG
5	NCOR1 F	GCATCGAGCTGCTGTTATCCC	62.9	145
	NCOR1 R	TCTCTGCCGCTGCTCCTCC
6	NCOR2 F	AAGCAGCGAGCGGCTGCCAT	72.6	143
	NCOR2 R	TGCTGAGGGGCGTCGCTCTC
7	FKBP4 F	GGGGACCGAGTCTTTGTCCACT	69.9	124
	FKBP4 R	CCCAAGCCTTGATGACCTCCC
8	MMP-2 F	GCCACATTCTGGCCTGAGCT	62.4	151
	MMP-2 R	CCAGGCTGGTCAGTGGCTTG
9	MMP-9 F	CTGGAGGTTCGACGTGAAG	55	155
	MMP-9 R	AACTCACGCGCCAGTAGAAG
10	TIMP3 F	CAAGTACCAGTACCTGCTGACA	55.4	122
	TIMP3 R	GATAGTTCAGCCCCTTGC
11	BMP5 F	CCTGAAGAGTCGGAGTACTCAG	59.2	139
	BMP5 R	GACTCTGGGTGGTCAGAGGA
12	CDH2 F	CATCCCTCCAATCAACTTGCC	59.2	131
	CDH2 R	GAGGCTGGTCAGCTCCTG
13	HOXA1 F	CTTCGCAGGACCAGGTCACTC	65.1	124
	HOXA1 R	GGTCCGAGGGGTAGGCTCG
14	IHH F	GCCGCGCGGTGGACATCA	71.9	126
	IHH R	CGGAGCAATGCACGTGGGCC
15	FKBP5 F	GGTGAAGCCCAGCTGCTC	66.7	114
	FKBP5 R	CTGGCACATGGAGATCTGC
16	PRMC1F	CGCCGCTCAACCTGCTGCT	69.7	139
	PRMC1 R	GTGAAGTCGCGCCGCTTGAG
17	ADAMTS 1 F	GCCGACTGGGAAAGCGGA	66.6	120
	ADAMTS 1 R	CCAGTTCCTGTGGGCTGTCC
18	WNT7A F	AGCTGGGCTACGTGCTCAAGG	68.2	138
	WNT7A R	CCAGGTCCGTGTCCATGGG
19	AREG F	GTTGCCCCAGAGACCGAGACG	72.6	113
	AREG R	CAGCATAATGGCCTGAGCCGA
20	L19 F	GCCAACTCCCGTCAGCAGA	60	154
	L19 R	TGGCTGTACCCTTCCGCTT

ISSN: 1477-7827