Figure 5
Changes in activation levels of MAPKs during LH/P 4 depletion and P 4 replacement treatments. Tissue lysates were prepared from CL of monkeys receiving VEH, CET (LH/P4 depletion) and CET+P4 (P4 replacement) treatments. The immunoblots shown are from one of three independent experiments (CL from one monkey at each time point was used per experiment). Immunoblots for phospho MAPKs and total MAPKs were analyzed by densitometry and the fold change in the protein levels of phospho/total MAPKs compared between treatments are represented numerically. β-actin was used as loading control.