Figure 4
Analysis of expression of genes associated with PR, its cofactors and P 4 target genes in CL of monkeys treated with VEH (5.25% glucose injection at mid luteal phase), CET (LH/P 4 depletion) and CET+P 4 (P 4 replacement). A) Real time RT-PCR expression analysis of various genes associated with PR signaling and its target genes. The fold expression change for VEH group was set as 1 and values for other groups were expressed in relation to the VEH group. For details on fold change calculation for real time RT-PCR analysis, see legend to Figure 1. Bars with different letters are significantly (P < 0.05) different for the individual genes examined. B) Immunoblot analysis of PR, NCOA1, NCOA2 and NCOA3 proteins in CL after LH/P4 depletion and P4 replacement. Immunoblots are analyzed by densitometry and densitometric value for VEH group is set as 1 fold and values for other groups were expressed in relation to the VEH group. For NCOA1 protein, the upper band indicated by asterisk was considered for densitometric analysis.