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Table 1 Primer sequences and PCR conditions

From: Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitromaturation and culture of bovine preimplantation embryos

Gene

Primer Sequence

Annealing Temperature

Product Length (bp)

GenBank n° of access/Reference

HSP70

F 5'AACAAGATCACCATCACCAACG3'

R 5'TCCTTCTCCGCCAAGGTGTTG3'

59°C

275

NM174550

BAX

F 5'TTTGCTTCAGGGTTTCATCCAGGA3'

R 5'CAGCTGCGATCATCCTCTGCAG3'

64°C

174

NM173894

ZAR1

F 5'TGCCGAACATGCCAGAAG3'

R 5'TCACAGGATAGGCGTTTGC3'

53°C

188

NM_001076203

MATER

F 5'TAATGACGACGCTGTGTTCTG3'

R 5'GCGGTTCTCAGGTTCTTCAG3'

53°C

206

NM_001007814

GDF9

F 5'GACCCCTAAATCCAACAGAA3'

R 5'AGCAGATCCACTGATGGAA3'

53°C

120

NM_174681

β - ACTIN (endogenous)

F 5'GACATCCGCAAGGACCTCTA3'

R 5'ACATCTGCTGGAAGGTGGAC3'

53°C

205

NM_173979

GAPDH (endogenous)

F 5'CCAACGTGTCTGTTGTGGATCTGA3'

R 5'GAGCTTGACAAAGTGGTCGTTGAG3'

53°C

237

MOUROT et al. [33]

  1. Primer sequences and PCR conditions used for relative gene expression analysis.