Analysis of ovarian receptors. (A) Representative western blotting analysis of androgen receptor (AR), estrogen receptor subunits (ER-α and ER-β) in rat ovaries after variation of maternal care. Indicated concentrations of each total protein (70 μg extracted from a pool of 6 organs/group) were used to detect specific protein expression levels in the blots (upper panel). (B) Densitometry values for AR, ER-α and ER-β levels were studied following normalization to the housekeeping gene β-actin. All results are expressed as means ± SEM (n = 6 animals/group). Student's t-test; * p < 0.05.