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Table 1 Derivation of the most immunodominant epitope of HSP90 beta to serve as a diagnostic marker

From: Anti-HSP90 autoantibodies in sera of infertile women identify a dominant, conserved epitope EP6 (380-389) of HSP90 beta protein

S.NO

PEPTIDES

SEQUENCE

MEAN ABSORBANCE

MEAN ABSORBANCE

CHANGE IN ABS

% INCREASE / DECREASE

RANK AS MARKER

   

PATIENT ± SD

CONTROL ± SD

[P -C / C X 100]

CHANGE IN ABSORBANCE

 

1

EP1

MPEEVHHGEEEV

0.89 ± 0.39

0.35 ± 0.12

154.2

54%

2

2

EP2

IPNPQERTLTLV

0.52 ± 0.24

0.26 ± 0.1

100

0.00%

6

3

EP3

ITKHNDDEQYA

0.83 ± 0.26

0.4 ± 0.15

107.5

8%

5

4

EP4

GSFTVRADHGEP

0.68 ± 0.24

0.34 ± 0.09

100

0%

6

5

EP5

YLEERRVKEVVK

0.51 ± 0.23

0.29 ± 0.08

75.86

-24.14%

8

6

EP6

VVDSEDLPLN

0.94 ± 0.33

0.35 ± 0.12

168.5

69%

1

7

EP7

RYHTSQSGDE

0.8 ± 0.32

0.4 ± 0.12

100

0.00%

6

8

EP8

GESKEQVANSA

0.78 ± 0.29

0.36 ± 0.09

116.6

17%

3

9

EP9

IDEYCVQQLK

0.34 ± 0.15

0.19 ± 0.06

78.94

-21%

7

10

EP10

NPDHPIVETL

0.73 ± 0.27

0.35 ± 0.14

108.5

8.50%

4

  1. Higher the value in the % increase in change of the absorbance, greater is the indication of the peptide to be the immunodominant epitope for that protein. Peptides giving a negative value (decrease change or no change) are not good candidates to be used as markers. Of all the 10 peptides, EP6 ranked first and showed the highest increase in % change in absorbance followed by EP1 and EP8.