Figure 6

Bioinformatics analyses of the immunodominant epitopes of HSP90β. Panel (A) shows pair wise alignment of sequences from human HSP90β (SwissProt Accession No: P08238) and yeast HSP90 (PDB ID: 2CG9). The regions of the predicted epitopes in the alignment are highlighted in boxes. Panel (B) The structural coordinates are missing for the EP1 peptide. The structure of yeast HSP90 (PDB ID: 2CG9) has been used to represent the structures of peptides EP6 and EP8. The predicted antigenic regions are colored based on their secondary structures. Helices, strands and loops are colored green, blue and pink respectively. Panel (C) shows the conservation of the 3 putative dominant peptides across various species. It can be clearly seen that peptide EP6 is highly conserved antigen in all the species and therefore is one of the potential immune targets of HSP90β. The structures were rendered using the online algorithm PyMOL.