Figure 4From: Anti-HSP90 autoantibodies in sera of infertile women identify a dominant, conserved epitope EP6 (380-389) of HSP90 beta proteinBiochemical characterization of epitope EP6. Panel (A) depicts dot blot analysis using rabbit polyclonal antibodies to peptides EP6. EP6 in panel A6 shows strong immunoreactivity with the post-immune sera. The pre-immune of the same rabbit did not react to any of the 10 peptides EP1 to EP10 in sequential order. Panel (B): Western blot analysis using rabbit polyclonal antibodies to peptide EP6 with recombinant HSP90 loaded in duplicates. B1: Pre immune serum shows no immunoreactivity. B2: Post immune serum reacts with the recombinant protein. Panel (C): Western blot analysis using the anti-EP6 peptide polyclonal antibodies with crude mice ovarian protein. Lane RO: total mice ovarian extract stained with Coomassie blue stain with an arrow indicating the locus representing the HSP90 protein. Lane Pre: The pre immune serum shows no immunoreactivity. Lane Post: The post-immune sera shows single band reactivity in the lane marked at the 90 kDa. Lane NC: Secondary alone control shows no reactivity.Back to article page