Figure 3

Dot blot analysis of patient's sera with immunodominant epitopes of HSP90β. Panels (A, B and C) are representative panels showing immunoreactivity of patients' sera testing positive in ELISA with EP6, EP1 and EP8 peptides respectively. Panel (D) shows no immunoreactivity to any of the 10 peptides probed with sera from normal fertile control serum. Panel (E1-10) demonstrates no reactivity of secondary antibody with any of the 10 peptides. Panel F1: Immunoreactivity of monoclonal to HSP90 with recombinant HSP90, serving as positive control for the assay. Panel F2: negative control for the monoclonal to HSP90. Panel (G and H): Immunoreactivity to 2 un-related peptides of Prostate Secretory Protein 94 - PSP94 (Panel G: KDPKKTCSVSE and panel H: KKEDCKYIVVEK) using both patient (G1, H1) and control (G2, H2) sera respectively.