Figure 2

EP6 is an immunodominant peptide of HSP90β. Epitopic peptides were selected by the epitope prediction algorithm by Kolaskar-Tongaonkar and Bepipred. These peptides were then custom synthesized using Fmoc chemistry and used in ELISA. The figure depicts the immunoreactivity of sera from infertile women with the 10 peptides. The solid horizontal bar indicates the 80^th centile of controls. Values above the bar indicate a positive immunoreactivity to that peptide and values below or on the bar depicts poor or no immunoreactivity. Peptide EP6 shows strong immunoreactivity with all the 64 sera tested in ELISA with no values falling below or on the bar. All outliers i.e. sera showing absorbance values of 2 and above were not used in statistical analysis and calculations to avoid ambiguity of results.