Figure 1

Putative epitopes of HSP90β, an ovarian specific isoform. A) Western blot analysis depicting the major isoform in total human ovarian extracts to be beta isoform as seen at the 90 kDa loci when probed with HSP90β antibody (lane 1). No immunoreactivity was seen with HSP90α antibody (lane 2). Mouse testicular extracts were used as positive control for the HSP90α antibody (lane 3). 'No primary/ secondary alone' antibodies served as negative control (lane 4). Antibody to GAPDH served as loading control to ensure equal amounts of protein per lane. B) The figure is a ClustalW2 alignment of the alpha and beta isoform of HSP90 and it maps the 10 short listed peptides (EP1-EP10) indicating its position relative to the isoforms.