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Table 2 Comparison of cell marker localization in isolated cells from ovaries

From: Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

In ovaries

Cell suspension

marker

Oo

IC

TC

EC

Ma

GC

IC

fibronectin

-

+

+

-

-

-

61.0% (891/1461)

laminin

-

+

+

+

-

-

88.1% (378/429)

Flk-1

-

-

-

+

-

-

0% (0/1500)

F4/80

-

-

-

-

+

-

0.3% (3/895)

  1. The staining of markers in the cells isolated from ovaries is shown as + (positive) or − (negative). The proportion of cells showing a positive signal in interstitial cell suspensions is expressed as a percentage. The numbers in parenthesis are the number of positive cells per total counted cells. Oo: oocyte, IC: interstitial cells, TC: theca cells, EC: endothelial cells, Ma: macrophages, and GC: granulosa cells.