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Table 1 Combination of the first and secondary antibodies and fixation conditions used in immunohistochemistry

From: Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

Antigen First antibody Secondary antibody Fixation
F4/80 rat anti-mouse F4/80 goat anti-rat IgG Alexa Fluor 488 4% PFA
Flk-1 goat anti-mouse Flk-1 donkey anti-goat IgG Alexa Fluor 488 4% PFA
fibronectin rabbit anti-mouse fibronectin goat anti-rabbit IgG biotinylated non-fixed
laminin rabbit anti-mouse laminin donkey anti-rabbit IgG Alexa Fluor 594 4% PFA
tenascin rat anti-mouse tenascin goat anti-rat IgG Alexa Fluor 488 4% PFA
collagen IV rabbit anti-mouse collagen IV donkey anti-rabbit IgG Alexa Fluor 594 4% PFA
THY1 rat anti-mouse THY1 goat anti-rat IgG Alexa Fluor 488 4% PFA
CYP17A-1 goat anti-mouse CYP17A-1 rabbi anti-goat IgG Alexa Fluor 594 Bouin's solution