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Table 1 Combination of the first and secondary antibodies and fixation conditions used in immunohistochemistry

From: Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

Antigen

First antibody

Secondary antibody

Fixation

F4/80

rat anti-mouse F4/80

goat anti-rat IgG Alexa Fluor 488

4% PFA

Flk-1

goat anti-mouse Flk-1

donkey anti-goat IgG Alexa Fluor 488

4% PFA

fibronectin

rabbit anti-mouse fibronectin

goat anti-rabbit IgG biotinylated

non-fixed

laminin

rabbit anti-mouse laminin

donkey anti-rabbit IgG Alexa Fluor 594

4% PFA

tenascin

rat anti-mouse tenascin

goat anti-rat IgG Alexa Fluor 488

4% PFA

collagen IV

rabbit anti-mouse collagen IV

donkey anti-rabbit IgG Alexa Fluor 594

4% PFA

THY1

rat anti-mouse THY1

goat anti-rat IgG Alexa Fluor 488

4% PFA

CYP17A-1

goat anti-mouse CYP17A-1

rabbi anti-goat IgG Alexa Fluor 594

Bouin's solution