Figure 5From: Evaluation of human first trimester decidual and telomerase-transformed endometrial stromal cells as model systems of in vitro decidualizationQuantitative mRNA expression of decidual marker genes upon cAMP stimulation or combined treatment with cAMP and E2P4 in differentiating HDSC and THESC. Cultures were incubated with E2P4 and/or cAMP for for 3, 6, 9 and 12 days and transcript levels were analysed by real-time PCR. For relative quantification of mRNA expression, cAMP-treated samples were arbitrarily set at 100%. Bars indicate mean values ± SEM of five and four different experiments performed in HESC and THESC, respectively. PCR reactions were done in duplicates. * indicates p < 0.05 compared to the cAMP-stimulated sample of the same day; n.s., not significant. A) PRL mRNA expression. B) IGFBP1 mRNA expression.Back to article page