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Table 4 Immunohistochemical localisation and staining intensities of the various components of the endocannabinoid system in the first trimester trophoblast and decidua.

From: Modulation of the endocannabinoid system in viable and non-viable first trimester pregnancies by pregnancy-related hormones

Antigen Cell type Surgical termination Medical termination Spontaneous
miscarriage
CB1 Syncytiotrophoblast
Cytotrophoblast
(Histoscore)
Mesenchymal core
(Histoscore)
Decidua
(Histoscore)
+/++
++
(161.0 ± 4.77)
-
(3.3 ± 0.40)
++
(66.6 ± 3.69)
+
+
(113.9 ± 7.48)
+
(15.5 ± 2.25)
++
(36.4 ± 5.57)
+
+
(79.8 ± 7.91)*
-
(8.8 ± 0.96)*
+
(15.0 ± 2.04)*
CB2 Syncytiotrophoblast
Cytotrophoblast
(Histoscore)
Mesenchymal core
(Histoscore)
Decidua
(Histoscore)
+
+
(96.5 ± 11.01)
-/+
(9.0 ± 1.96)
+
(14.8 ±1.59)
+/++
+
(114.3 ± 16.40)
-/+
(14.3 ± 2.4)
+/++
(68.8 ± 8.04)
++
++
(233.5 ± 5.27)*
++
(223.5 ± 5.27)**
+/++
(78.7 ± 6.06)ns
NAPE-PLD Syncytiotrophoblast
Cytotrophoblast
(Histoscore)
Mesenchymal core
(Histoscore)
Decidua
(Histoscore)
-/+
+
(9.4 ± 2.77)
-
(1.7 ± 0.56)
-/+
(4.9 ± 0.51)
+/++
+
(32.0 ± 2.45)
-
(2.0 ± 0.23)
+
(11.2 ± 1.62)
-/+
+/++
(14.2 ± 2.68)ns
-
(1.3 ± 0.38)ns
-/+
(4.6 ± 0.69)*
FAAH Syncytiotrophoblast
Cytotrophoblast
(Histoscore)
Mesenchymal core
(Histoscore)
Decidua
(Histoscore)
+
-/+
(45.5 ± 7.08)
++
(42.0 ± 6.02)
+
(13.9 ± 2.34)
+/++
++
(127.8 ± 6.26)
+
(22.7 ± 5.03)
-/+
(23.2 ± 6.38)
+
+
(105.2 ± 7.74)ns
-
(2.6 ± 0.59)*
++
(55.3 ± 4.00)**
  1. Scoring system: (-) = absent; (+) = staining visible; (-/+) = staining occasionally absent;
  2. (++) = stronger staining. The histoscore value for each antigen is shown as (mean ± sem) under each tissue that it represents. Each histoscore consists of 10 random fields/slide, for 10 matched samples each of surgical termination, medical abortion and spontaneous miscarriages taken from weeks 7 and 8 of gestation, as described in the Materials & Methods section. Data in bold are significantly different to respective surgical termination tissue and *P<0.01;**P<0.001; One-way ANOVA with Bonferroni's ad hoc post-test when compared to the medical abortion group.