Figure 6From: Expression of aldo-keto reductase family 1 member C1 (AKR1C1)gene in porcine ovary and uterine endometrium during the estrous cycle and pregnancyImmunoblot analysis of AKR1C1 recombinant protein in CHO-K1 cells. The bands corresponding to the protein produced by pcDNA3 + pAKR1C1 and pcDNA4/HisMax + pAKR1C1 vectors were detected. After gel electrophoresis, the proteins were transferred to a nitrocellulose membrane. The protein on the blot was detected with rabbit anti-bovine 20α-HSD, followed by staining with anti-rabbit IgG-POD. Lane 1, pcDNA3 + pAKR1C1; lane 2, pcDNA4/HisMax + pAKR1C1.Back to article page