Figure 2

AKR1C1 mRNA expression in the ovary during the estrous cycle. A. AKR1C1 mRNA was detected by RT-PCR. B. Real-time PCR results. Ovarian tissues were obtained by laparotomy under general anesthesia on days 0, 5, 10, and 15 of the estrous cycle. Total RNA was extracted and then subjected to RT-PCR and real-time PCR. The amplified products of the AKR1C1 and GAPDH genes were separated on agarose gel and stained with ethidium bromide. Representative results are shown; graphs show the average ± SEM of 3 independent experiments.