Expression and quantitation of A2M mRNA in normal and cancerous ovaries from hens. [A] RT-PCR analyses were performed using cDNA templates from normal and cancerous ovaries of chickens with chicken A2M and GAPDH-specific primers. Lanes 1 to 4 show results of analysis of four normal ovaries with distilled water as a negative control. [B] Lanes 1-10 are from analyses of 10 different cancerous ovaries. Expression of A2M mRNA was predominantly in endometrioid carcinoma, with little or no expression in serous, mucinous or clear cell carcinomas and normal ovaries (mean ± SEM; P < 0.05). Legend for panel B: Lane 1, clear cell carcinoma (Stage IV); Lane 2, endometrioid/serous/mucinous carcinoma (Stage III); Lane 3, endometrioid carcinoma (Stage I); Lane 4, serous carcinoma (Stage I); Lane 5, mucinous/endometrioid carcinoma (Stage IV); Lane 6, endometrioid carcinoma (Stage IV); Lane 7, endometrioid carcinoma (Stage III); Lane 8, clear cell carcinoma (Stage IV); Lane 9, serous/mucinous carcinoma (Stage IV); and Lane 10, serous/mucinous/endometrioid carcinoma (Stage III).