RT-PCR and in situ hybridization analyses of A2M mRNAs in the chicken oviduct. Results of RT-PCR analysis using cDNA from different segments from female chicken [A] indicate that A2M mRNA is expressed abundantly in the infundibulum, magnum, and isthmus of the oviduct. At lower abundance, A2M mRNA is expressed in shell gland. [B] Cross-sections of the infundibulum, magnum, isthmus and shell gland of the chicken oviduct hybridized with antisense or sense chicken A2M cRNA probes revealed that A2M mRNA is most abundant in the glandular epithelium (GE) of the magnum, and lesser abundant in GE of the isthmus and luminal epithelium (LE) and GE of the shell gland. Legend: LE, luminal epithelium; GE, glandular epithelium; Scale bar represents 200 μm (the first columnar panels) and 50 μm (the second columnar panels).