Figure 5
Dose-response effect of estrogenic mycotoxins and 17β-estradiol on proportion of live acrosome-reacted stallion sperm. Exposure to α- and β-ZOL at a concentration of 0.1 mM significantly increased the percentage of (live) acrosome reacted sperm (p < 0.0001 and p < 0.05, respectively), when compared to controls ("0 μM"). No effects of ZEA or E2 on the percentage of live acrosome reacted cells were observed. Different letters indicate values that differ significantly a vs b = p < 0.05; a vs c = p < 0.001; n = 5.