Figure 4
Dose-response effect of estrogenic mycotoxins and 17β-estradiol on linearity (LIN) of stallion sperm. In vitro exposure to 0.1 mM α-ZOL, β-ZOL and E2 significantly reduced LIN (p < 0.0001; p < 0.05 and p < 0.05, respectively), compared to control ("0 μM"), whereas ZEA did not modify this parameter at any examined concentration. Least Square Means obtained by the mixed model procedure. Different letters indicate values that differ significantly a vs b = p < 0.05; a vs c = p < 0.001; n = 5, replicates = 3.