Figure 3
From: Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm
Acrosome reaction in leptin-treated pig sperm. (A) Representative FITC-PNA fluorescence pattern of spermatozoa incubated with 10 nM leptin. Scale bars: 5 μm. (B) Incidence of positive acrosome reaction in control spermatozoa, spermatozoa incubated with L-NAME, spermatozoa incubated with 10 nM leptin, spermatozoa incubated with 10 nM leptin in presence of L-NAME and spermatozoa incubated with 10 nM leptin+ Ab-ObR. Values are mean percentage ± SD (** p < 0.01).