Figure 3

Alternate Pate-C and Pate-M transcripts A: A schematic figure of the Pate-C genomic locus and the detected Pate-C transcripts (A.1-A.3). Exon and intron lengths are not drawn to scale. B: A schematic figure of the Pate-M genomic locus and of the detected Pate-M transcripts (B.1-B.4). Exon and intron lengths are not drawn to scale. C: Northern hybridization of epididymal RNA with Pate-C specific probe revealing the presence of two transcripts. D: RT-PCR analysis of epididymal RNA with Pate-M specific primers as indicated in B. The longer PCR products reveal transcripts including exons 6 (top) and 10 (bottom), whereas the shorter products indicate their absence. The transcript sequences were further confirmed by sequencing the PCR products (not shown).