Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos

Table 2 In vitro development potential and quality of bovine presumed zygotes

Culture system	Presumed zygote (n)	% cleaved (range in %)	% blastocyst (range in %)	Total blastomeres (range)	% apoptotic blastomeres (range in %)
ControlOPU	201	64.3^a ± 32.0 (0-100)	13.9^a ± 18.1 (0-57.1)	118.3^a ± 24.4 (65-163)	4.6^a ± 2.0 (1.3-7.5)
Agar OPU^x	151	82.8^a± 26.1 (0-100)	35.2^b ± 26.3 (0-100)	128.2^a ± 29.5 (73-215)	4.9^a ± 1.6 (2.1-7.8)
s IVP^y	120	74.0^a ± 11.6 (53.3-100)	30.8^b ± 13.8 (0-50.0)	123.1^a ± 28.9(65-188)	3.8^a ± 2.7 (1-10.8)
dmapIVP^z	143	72.8^a ± 5.0 (67.3-76.9)	28.6^b ± 3.9 (25.0-32.7)	124.2^a ± 36.7 (65-175)	5.3^a ± 2.8 (1.2-11.0)

Cleavage and blastocyst development rates were calculated based on total presumed zygotes transferred into IVC medium.
^{a, b} Values with different superscript letters within a column differ significantly (P < 0.01).
^x Development rates are those of OPU cultured zygotes. The development rates of agar-embedded slaughterhouse embryos were not considered.
^y A group of 10 COCs from slaughterhouse ovaries was used for IVP.
^z Slaughterhouse COCs pre-treated with 2 mM 6-dimethylaminopurine for 6~7 h before maturation followed by IVM, IVF and IVC.

ISSN: 1477-7827