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Table 2 In vitro development potential and quality of bovine presumed zygotes

From: Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos

Culture system Presumed zygote (n) % cleaved
(range in %)
% blastocyst
(range in %)
Total blastomeres
(range)
% apoptotic blastomeres
(range in %)
ControlOPU 201 64.3a ± 32.0 (0-100) 13.9a ± 18.1 (0-57.1) 118.3a ± 24.4 (65-163) 4.6a ± 2.0 (1.3-7.5)
Agar OPUx 151 82.8a± 26.1 (0-100) 35.2b ± 26.3 (0-100) 128.2a ± 29.5 (73-215) 4.9a ± 1.6 (2.1-7.8)
s IVPy 120 74.0a ± 11.6 (53.3-100) 30.8b ± 13.8 (0-50.0) 123.1a ± 28.9(65-188) 3.8a ± 2.7 (1-10.8)
dmapIVPz 143 72.8a ± 5.0 (67.3-76.9) 28.6b ± 3.9 (25.0-32.7) 124.2a ± 36.7 (65-175) 5.3a ± 2.8 (1.2-11.0)
  1. Cleavage and blastocyst development rates were calculated based on total presumed zygotes transferred into IVC medium.
  2. a, b Values with different superscript letters within a column differ significantly (P < 0.01).
  3. x Development rates are those of OPU cultured zygotes. The development rates of agar-embedded slaughterhouse embryos were not considered.
  4. y A group of 10 COCs from slaughterhouse ovaries was used for IVP.
  5. z Slaughterhouse COCs pre-treated with 2 mM 6-dimethylaminopurine for 6~7 h before maturation followed by IVM, IVF and IVC.