Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos

Table 1 Primers used for quantitative real-time PCR analysis

Gene name	GeneBank accession number	Forward primer (5'-3')	Reverse primer (5'-3')	Product length (base pairs)
AKR1B1	BT021058	TGCAACCCAAATACTCTTTT	AAAAGCCTAGCTGAAAGGAT	106
ATP5A1	M_174684.2N	GACCGATGGAAAGATCTCAG	AACCACCAAGCAACATGG	212
CD9	NM_173900	AGATCTTCCGAAGCAAATTC	CAAAGTTAGTGGCAAAGGAA	238
ODC1	NM_174130.2	CTATGTGATGTCAGGGCCAA	CACGTTAATACGCGTGGAAG	166
PGSH2	NM_174445	ATCCTCCCACAGTCAAAGAT	GCACATCACACACTCTGTTG	162
PLAC8	NM_001025325	TCTGACATTTTTACCGCTCT	ATTTCATTGCAGCATTTTCT	131
PLAU	NM_174147.2	ATCCCTCTGACTATCGCTAC	CAGGAATCTGTTTCCCACTG	151
TGFβ1	NM_001166068.1	TGGAGCTGTACCAGAAATATAGCAA	GCCACTGCCGCACAACTC	120
TXN	AF104105	TGATCAAGCCTTTCTTTCAT	TAATGGTGGCTTCAAGTTTT	195

AKR1B1: aldo-keto reductase family 1, member B1; ATP5A1: alpha subunit ATP synthase isoform 5 A1; CD9: CD9 molecule; ODC1: ornithine decarboxylase; PLAU: urokinase-type plasminogen activators; PGSH2: prostaglandin G/H synthase-2; PLAC8: placenta-specific 8; TGFβ1: transforming growth factor beta 1; TXN: thioredoxin.
Primer sequences for 18s ribosomal RNA (18srRNA), beta actin (ACTB), caspase3 (CASP3), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) were published previously [25].

ISSN: 1477-7827