MDDCs secrete α-defensins 1-3: E2 and PG effect. A) ELISA quantification of a-defensins 1-3 secreted by MDDCs, mDCs and pDCs (left panel) or PBMCs and monocytes (right panel) in the supernatant after 6 h of culture. Data are presented as mean plus or minus SD from 3 independent experiments.*P < 0.01 and *P < 0.001 versus MDDCs levels. Data are presented in a log scale. Levels of α-defensins 1-3 measured by ELISA in: (B) MDDCs treated for 24 h with 10, 1 and 0.1 ug/ml of E2 (left panel) or PG (right panel). (C) MDDCs pretreated for 24 h with E2 or PG (10 ug/ml) before NDV-B1 infection for 10 h. Data are presented as mean plus or minus SD from 3 independent experiments. *P < 0.05versus MDDCs levels.