Double immunohistochemistry representing the colocalisation of p97/VCP and Jab1/CSN5 in the developing rat testis (A-D). Detection of Jab1/CSN5 (green), p97/VCP (red), and double-stained (yellow) positive cells merged with DAPI nuclear staining on day 5 (A), day 15 (B), day 30 (C) and day 60 (D). A: Day 5 gonocytes (GC) are double labelled with p97/VCP and Jab1/CSN5. B: Double-labelled spermatocytes (Sc), spermatogonia (Sg) and Sertoli cells (Se) are shown on day 15, and the nuclear regions of some spermatocytes are only positive for Jab1/CSN5. C: Jab1/CSN5 and p97/VCP positivity (yellow) is observed in spermatocytes (Sc), spermatogonia (Sg), Sertoli cells (Se) and elongating spermatids (eSt) on day 30. D: Double staining (yellow) is detected in the cytoplasm of spermatocytes (Sc), the nuclear region of spermatogonia (Sg) and elongating spermatids (eSt) and Sertoli cells (Se) in the 60-day-old rat testis. Scale bars: 25 μm.