Figure 2
Immunohistochemical analysis of Sertoli cells at day 5 (A), 15 (B), 60 (C) and colocalisation of p97/VCP - p27kip1 (D-F) and Jab1/CSN5 - p27kip1 (G-I) in rat testis. Cytokeratin 18 (CK) are detected in immature Sertoli cells (Se) at day 15 (A) and p27kip1 is observed in mature Sertoli cells (Se) of day 30 and 60 (B, C) in rat testis. Mature Sertoli cells (Se) are immunopositive for p27kip1 (green, D, G), p97/VCP (red, E) and Jab1/CSN5 (red, H), resulting in a yellow color (overlay, F, I). Scale bars: 25 μm.