Table 1 Specificity of IFMAs for hCG and/or hCG-variants
From: Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis
Assay type | Assay specificity | Capture mAb Epitope localization | Detection mAb Epitope localization |
|---|---|---|---|
hCG | hCG intact αβ heterodimer, bioactive; hCGn nicked αβ heterodimer, nicks in the region of aa hCGβ44-48 | INN-hCG-45 c3 hCGβ loop 2 | INN-hCG-22 β2 hCGβ loops 1+3 aa 20-25+68-77 |
hCGβ | hCGβ intact non-combined free hCGβ-subunit, aa hCGβ1-145; hCGβcf core fragment of hCGβ; aa hCGβ6-40 linked to hCGβ55-92; hCGβn nicked hCGβ, nicks in the region of aa hCGβ44-48 | INN-hCG-68 β7 hCGβcf | INN-hCG-22 |
hCGβcf | hCGβcf-only | INN-hCG-106 β11 hCGβcf | INN-hCG-22 |
hCGα | hCGα intact non-combined free a-subunit of hCG; aa hCGα1-92 | INN-hCG-72 α6 hCGα 33-42 | INN-hFSH-158 α5 Loop 3 (Tyr 65) |