Induction of ovulation in Xenopus without hCG injection: the effect of adding steroids into the aquatic environment

Table 1 Incorporation of steroids into tissues of Xenopus

Steroids		Progesterone			Estradiol
Added ³H-steroid (dpm/g)		477,700 ± 8,190			293,600 ± 39,240
Dissolved in 0.1 M NaCl (dpm/g)		3,943 ± 438			3,777 ± 681
Estimated concentration (μM)		0.17 ± 0.02			0.13 ± 0.02
Tissues	Blood	Ovary	Muscle	Blood	Ovary	Muscle
Incorporation into tissues (dpm/g)	17,180 ± 557	38,380 ± 5,252	14,920 ± 1,670	4,671 ± 365	4,280 ± 1,080	2,236 ± 328
Estimated concentration (μM)	0.72 ± 0.02	1.61 ± 0.22	0.62 ± 0.07	0.16 ± 0.01	0.15 ± 0.04	0.08 ± 0.01

Each value in the mean (± S.D.) of three separate experiments using samples of three separate bottles or females