Analysis of ovarian receptors. (A) Representative Western blotting analysis of androgen receptor (AR), estrogen receptor (ER-α and ER-β), progesterone receptor (PRA and PRB) and melatonin receptor (MT1R) in ovarian tissue of rats receiving melatonin [100 μg/100 g B.W]. Indicated concentrations of each total protein (70 μg extracted from a pool of 6 organs/group) were used to detect specific protein expression levels in the blots (upper panel). (B) Densitometry values for AR, ER-α, ER-β, PRA, PRB and MT1R levels were studied following normalization to the house-keeping gene (β-actin). All results are expressed as mean ± SEM (N= 6 animals/group). * p < 0.05 vs. control group.