Table 1 Antibodies used in Western blot analysis
From: Altered placental development in undernourished rats: role of maternal glucocorticoids
Primary antibodies and their commercial source | First antibody dilution | Horse radish peroxidase-linked secondary antibodies, and their commercial source | Positive controls | Secondary antibody dilution |
|---|---|---|---|---|
Rabbit polyclonal HSD11B-1 (Cayman Ann Arbor, MI) | 1:500 | Rabbit immunoglobulin G (Millipore, | Rat liver tissue lysate | 1:2500 |
Sheep polyclonal HSD11B-2 (Chemicon, Temecula, CA) | 1:4000 | Sheep immunoglobulin G (Upstate, Temecula, CA) | Rat liver tissue lysate | 1:5000 |
Rabbit polyclonal GR (Abcam, Cambridge, MA) | 1:3500 | Rabbit immunoglobulin G (Millipore, MA) | Rat liver tissue lysate | 1:2500 |
Rabbit polyclonal SLA2C1 (Santa Cruz, CA) | 1:700 | Rabbit immunoglobulin G (Millipore, MA) | H4 cell lysate (Santa Cruz) | 1:2500 |
Rabbit polyclonal SLA2C3 (Millipore Corp., CA) | 1:700 | Rabbit immunoglobulin G (Millipore Corp., Ca) | SLAC2A3 peptide (Millipore) | 1:2500 |
Rabbit polyclonal SLC38A1 (Santa Cruz, CA) | 1:700 | Rabbit immunoglobulin G (Millipore Corp., MA) | 293T cell lysates (Santa Cruz) | 1:2500 |
Rabbit polyclonal SLC38A2 (Santa Cruz, CA) | 1:700 | Rabbit immunoglobulin G (Millipore Corp., MA) | U-87MG cell lysates (Santa Cruz | 1:2000 |
Rabbit polyclonal SLC38A4 (Santa Cruz, CA) | 1:700 | Rabbit immunoglobulin G (Millipore Corp., MA) | 293 T cell lysate (Santa Cruz) | 1:2000 |