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Table 1 Antibodies used in Western blot analysis

From: Altered placental development in undernourished rats: role of maternal glucocorticoids

Primary antibodies and their commercial source First antibody dilution Horse radish peroxidase-linked secondary antibodies, and their commercial source Positive controls Secondary antibody dilution
Rabbit polyclonal
HSD11B-1 (Cayman Ann Arbor, MI)
1:500 Rabbit immunoglobulin G
(Millipore,
Rat liver tissue lysate 1:2500
Sheep polyclonal
HSD11B-2 (Chemicon,
Temecula, CA)
1:4000 Sheep immunoglobulin G
(Upstate, Temecula, CA)
Rat liver tissue lysate 1:5000
Rabbit polyclonal
GR (Abcam, Cambridge, MA)
1:3500 Rabbit immunoglobulin G
(Millipore, MA)
Rat liver tissue
lysate
1:2500
Rabbit polyclonal
SLA2C1 (Santa Cruz, CA)
1:700 Rabbit immunoglobulin G (Millipore, MA) H4 cell lysate (Santa Cruz) 1:2500
Rabbit polyclonal
SLA2C3 (Millipore Corp., CA)
1:700 Rabbit immunoglobulin G
(Millipore Corp., Ca)
SLAC2A3 peptide (Millipore) 1:2500
Rabbit polyclonal
SLC38A1 (Santa Cruz, CA)
1:700 Rabbit immunoglobulin G
(Millipore Corp., MA)
293T cell lysates
(Santa Cruz)
1:2500
Rabbit polyclonal
SLC38A2 (Santa Cruz, CA)
1:700 Rabbit immunoglobulin G
(Millipore Corp., MA)
U-87MG cell lysates (Santa Cruz 1:2000
Rabbit polyclonal
SLC38A4 (Santa Cruz, CA)
1:700 Rabbit immunoglobulin G
(Millipore Corp., MA)
293 T cell lysate
(Santa Cruz)
1:2000