Figure 4

IL-1β-induced downregulation of the decidualization capacity of KC02-44D cells. KC02-44D cells were pretreated with IL-1β (1.0 ng/ml) for 4 days and then stimulated with the indicated combinations of 10^-8 M E2, 10^-6 M MPA, and 0.125 mM dibutyryl cAMP for 7 days. (a) Expression of decidualization marker IGFBP-1 in KC02-44D cells at passage number 19 (ELISA). The data represent the mean ± SEM of triplicate measurements. *P < 0.05, *P < 0.01 and ***P < 0.001 by Tukey's test. (b) Expression of IGFBP-1 and the PR in KC02-44D cells at passage number 16 (Western blot analysis). β-Actin was used for normalization. † = nonspecific band.