Table 1 Porcine primer pair characteristics
From: Examination of relaxin and its receptors expression in pig gametes and embryos
Gene names | GenBank Acc. (NCBI) | Primer sequences (5'-3') | AT (oC) | Amplicon sizes (bp) |
|---|---|---|---|---|
RXFP1 | CA994862.1 | S: AGGCTGACGAGGACAACT | 52.5 | 132 |
| Â | Â | AS: CAGAACCGACCAAGCATT | Â | Â |
RXFP2 | CA997681.1 | S: CATCTGCTGGATTCCCGTAT | 55 | 117 |
| Â | Â | AS: TTCAAGGCACTGTTCACC | Â | Â |
RELAXIN | NM213872.1 | S: TGTGGCTCCGTCTCCTGGGG | 55 | 164 |
| Â | Â | AS: GTTGCCTTCAGCTCCTGTGGC | Â | Â |
β-ACTIN | U07786 | S: 5'-ACTGGCATTGTCATGGACTCTG-3' | 60 | 397 |
| Â | Â | AS: 5'-AGTTGAAGGTGGTCTCGTGGAT-3' | Â | Â |