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Table 1 Primers used for RT-PCR and qRT-PCR

From: Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placenta

Primer

GenBank Accession no.

Forward 5'-3'

Reverse 5'-3'

PCR(bp) product

T °C

Mif

NM_001111330

TGCCCAGAACCGCAACTACAGTAA

TCGCTACCGGTGGATAAACACAGA

218

60

Cyclophilin

NM_177832.3

CTTGCTGCAGACATGGTC

GCAATCCTGCTAGACTTG

660

58

β-actin

NM_007393

CTGTGGCATCCACGAAACTA

AGTACTTGCGCTCAGGAGGA

199

60

  1. Sequences of oligonucleotide primers, their annealing temperatures (T) and expected product sizes