Figure 3

PCR analysis of Mif mRNA in ectoplacental cone and placentas at different gestational periods. Panel A. Representative agarose gel photograph showing ethidium bromide-stained RT-PCR products of Mif and cyclophilin genes (internal control, cyclophilin). 1 and 2, 7.5 gd; 3 and 4, 10.5 gd; 5 and 6, 13.5 gd; 7 and 8, 17.5 gd. Panel B. Mif mRNA expression using RT-qPCR. Mif mRNA levels relative to β-actin were measured in all gestational periods indicated using RT-qPCR; the values are given as means ± SEM of three independent experiments. All comparisons were two-tailed. The RT-qPCR results revealed that placentas from gd10.5 show significant up-regulation (*) of Mif mRNA levels compared with gd7.5 (p = 0.054), 13.5 (p = 0.048) and 17.5 (p = 0.009).