Figure 3From: Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placentaPCR analysis of Mif mRNA in ectoplacental cone and placentas at different gestational periods. Panel A. Representative agarose gel photograph showing ethidium bromide-stained RT-PCR products of Mif and cyclophilin genes (internal control, cyclophilin). 1 and 2, 7.5 gd; 3 and 4, 10.5 gd; 5 and 6, 13.5 gd; 7 and 8, 17.5 gd. Panel B. Mif mRNA expression using RT-qPCR. Mif mRNA levels relative to β-actin were measured in all gestational periods indicated using RT-qPCR; the values are given as means ± SEM of three independent experiments. All comparisons were two-tailed. The RT-qPCR results revealed that placentas from gd10.5 show significant up-regulation (*) of Mif mRNA levels compared with gd7.5 (p = 0.054), 13.5 (p = 0.048) and 17.5 (p = 0.009).Back to article page