Figure 2From: Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placentaWestern blotting of ectoplacental cone (gd7.5) and placental (gds 10.5, 13.5 and 17.5) homogenates. The top panel (A) is a representative Western blot showing the detection of MIF protein at different gestational days (numbers on the top). Equal amounts of total cell lysate protein from each sample were separated by SDS-PAGE and immunodetected by Western blotting using anti-mouse MIF antibody. Equivalence of protein loading was confirmed by Ponceau S staining (lower bands). Panel B shows the MIF level by densitometry, presented as mean ± SD of three samples from three separate experiments. *P < 0.05 in relation to the gd7.5.Back to article page