Figure 3

Analysis of DERL1 mRNA expression during follicular development by semi-quantitative RT-PCR. The control gene was GAPDH and showed no significant differences in mRNA expression among the different groups. The DERL1 PCR signals were normalized with their corresponding GAPDH signals, and the results are presented as a relative change in ratio among groups. Different letters denote samples that differed significantly (p < 0.05) when Tukey-Kramer multiple comparison tests were performed to compare group means. Data are presented as means ± SEM. DF = dominant follicles (n = 4); OF = ovulatory follicles (n = 4); SF = 2-4 mm small follicles (n = 3); CL = corpus luteum collected on day 5 of the estrous cycle (n = 3).